Christopher Daniel Barnes

Peter Parker / Spider-Man (voice)

Sara Ballantine

Mary Jane Watson (voice)

Efrem Zimbalist Jr.

Doctor Octopus (voice)

Neil Ross

Norman Osborn / Green Goblin (voice)

Ed Asner

J. Jonah Jameson (voice)

Gary Imhoff

Harry Osborn (voice)

Joan Lee

Madame Web (voice)

Dimitra Arliss

Anastasia Hardy (voice)

Linda Gary

Aunt May (voice)

Jennifer Hale

Felicia Hardy (voice)

Michael Des Barres

Jackson Weele (voice)

Majel Barrett

Anna Watson (voice)